Review



myosin heavy chain isoforms  (Developmental Studies Hybridoma Bank)


Bioz Verified Symbol Developmental Studies Hybridoma Bank is a verified supplier
Bioz Manufacturer Symbol Developmental Studies Hybridoma Bank manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95

    Structured Review

    Developmental Studies Hybridoma Bank myosin heavy chain isoforms
    Myosin Heavy Chain Isoforms, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 95/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/myosin+isoforms/anti-Myosin+heavy+chain/pmc12985366-150-8-27
    Average 95 stars, based on 18 article reviews
    myosin heavy chain isoforms - by Bioz Stars, 2026-09
    95/100 stars

    Images

    Related Articles

    Incubation:

    Article Title: Locomotor Training with Adjuvant Testosterone Preserves Cancellous Bone and Promotes Muscle Plasticity in Male Rats after Severe Spinal Cord Injury
    Article Snippet: Membranes were stained with Ponceau S, imaged using a CCD digital imager (AI600, Amersham, Madison, WI), de-stained with tris-buffered saline with 0.01%-Tween 20 (TBST), and blocked at room temperature for 60-min in 5% w/v bovine serum albumin (BSA)-TBST solution. .. Membranes were incubated overnight at 4°C with primary antibody in 1% BSA-TBST, as follows: 1:1000 for PGC-1α and PGC-1β (Abcam, #ab54481, RRID:AB_881987 and #ab176328) and 1:500 for the myosin isoforms (type I: #BA-D5, RRID:AB_2235587; type IIa: #2F7, RRID:AB_1157865; type IIx: #6H1, RRID:AB_1157897; DSHB, Iowa City, IA]. .. The next morning, membranes were washed 3×10-min in TBST and placed in anti-rabbit (#7074, RRID:AB_2099233) or anti-mouse (#7076, RRID:AB_330924) horseradish peroxidase (HRP)-linked secondary antibody (Cell Signaling) for 60-min at room temperature.

    Pyrolysis Gas Chromatography:

    Article Title: Locomotor Training with Adjuvant Testosterone Preserves Cancellous Bone and Promotes Muscle Plasticity in Male Rats after Severe Spinal Cord Injury
    Article Snippet: Membranes were stained with Ponceau S, imaged using a CCD digital imager (AI600, Amersham, Madison, WI), de-stained with tris-buffered saline with 0.01%-Tween 20 (TBST), and blocked at room temperature for 60-min in 5% w/v bovine serum albumin (BSA)-TBST solution. .. Membranes were incubated overnight at 4°C with primary antibody in 1% BSA-TBST, as follows: 1:1000 for PGC-1α and PGC-1β (Abcam, #ab54481, RRID:AB_881987 and #ab176328) and 1:500 for the myosin isoforms (type I: #BA-D5, RRID:AB_2235587; type IIa: #2F7, RRID:AB_1157865; type IIx: #6H1, RRID:AB_1157897; DSHB, Iowa City, IA]. .. The next morning, membranes were washed 3×10-min in TBST and placed in anti-rabbit (#7074, RRID:AB_2099233) or anti-mouse (#7076, RRID:AB_330924) horseradish peroxidase (HRP)-linked secondary antibody (Cell Signaling) for 60-min at room temperature.

    Western Blot:

    Article Title: An optimized procedure for isolation of rodent and human skeletal muscle sarcoplasmic and myofibrillar proteins
    Article Snippet: .. Western blotting for myosin and actin detection was performed using an anti-myosin antibody against all myosin isoforms (DSHB, Iowa City, IA, USA; Cat. # A4.1025) and an anti-actin antibody against actin (α-sarcomeric) (Sigma-Aldrich, Saint Louis, MO, USA; Cat. # A2172), respectively, per the methods of Zergeroglu et al . [ ]. .. Briefly, SDS-PAGE was performed on SDS-PAGE sample preps from MF protein resuspensions, and proteins were transferred to polyvinylidene difluoride membranes at 200 mA for 2 h (Bio-Rad Laboratories).

    Immunolabeling:

    Article Title: Early microgliosis precedes neuronal loss and behavioural impairment in mice with a frontotemporal dementia-causing CHMP2B mutation
    Article Snippet: .. Fibre-type-specific myosin isoforms were immunolabeled with a combination of the following monoclonal antibodies: clone BF-F3 (type-IIb), clone BF-35 (all fibers except type-IIx) both from Developmental Studies Hybridoma Bank, and immunofluorescently labelled using goat anti-mouse IgM-AlexaFluor488, goat anti-mouse IgG-AlexaFluor568, and Wheat Germ Agglutinin (WGA)-Alexa633 (from Invitrogen). ..

    Bioprocessing:

    Article Title: Early microgliosis precedes neuronal loss and behavioural impairment in mice with a frontotemporal dementia-causing CHMP2B mutation
    Article Snippet: .. Fibre-type-specific myosin isoforms were immunolabeled with a combination of the following monoclonal antibodies: clone BF-F3 (type-IIb), clone BF-35 (all fibers except type-IIx) both from Developmental Studies Hybridoma Bank, and immunofluorescently labelled using goat anti-mouse IgM-AlexaFluor488, goat anti-mouse IgG-AlexaFluor568, and Wheat Germ Agglutinin (WGA)-Alexa633 (from Invitrogen). ..

    Whole Genome Amplification:

    Article Title: Early microgliosis precedes neuronal loss and behavioural impairment in mice with a frontotemporal dementia-causing CHMP2B mutation
    Article Snippet: .. Fibre-type-specific myosin isoforms were immunolabeled with a combination of the following monoclonal antibodies: clone BF-F3 (type-IIb), clone BF-35 (all fibers except type-IIx) both from Developmental Studies Hybridoma Bank, and immunofluorescently labelled using goat anti-mouse IgM-AlexaFluor488, goat anti-mouse IgG-AlexaFluor568, and Wheat Germ Agglutinin (WGA)-Alexa633 (from Invitrogen). ..



    Similar Products

    95
    Developmental Studies Hybridoma Bank myosin heavy chain isoforms
    Myosin Heavy Chain Isoforms, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/myosin+isoforms/anti-Myosin+heavy+chain/pmc12985366-150-8-27
    Average 95 stars, based on 1 article reviews
    myosin heavy chain isoforms - by Bioz Stars, 2026-09
    95/100 stars
      Buy from Supplier

    94
    Cell Signaling Technology Inc cell signaling phospho mlc rabbit
    Cell Signaling Phospho Mlc Rabbit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/myosin+isoforms/Myosin+Light+Chain+2v+(Cardiac+Isoform)+Rabbit+mAb/pm41905730-356-39-39
    Average 94 stars, based on 1 article reviews
    cell signaling phospho mlc rabbit - by Bioz Stars, 2026-09
    94/100 stars
      Buy from Supplier

    96
    Developmental Studies Hybridoma Bank anti myhc
    Anti Myhc, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/myosin+isoforms/anti-Myosin+all+isoforms/pmc13140482-87-4-5
    Average 96 stars, based on 1 article reviews
    anti myhc - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    96
    Developmental Studies Hybridoma Bank primary antibodies against myosin heavy chain isoforms
    Baseline morphological and physiological characteristics of the mice following HRW intake. (A) Body weight (g) of the mice consuming purified water (PW) or hydrogen-rich water (HRW) (PW: n = 12 per group, HRW: n = 14 per group). (B) Forelimb grip strength (g/g), measured weekly during the intervention period (PW: n = 12 per group, HRW: n = 14 per group). (C) Wet masses of six hindlimb skeletal muscles (soleus, extensor digitorum longus (EDL), plantaris, tibialis cranialis (TC), gastrocnemius, and quadriceps femoris) (PW: n = 12 per group, HRW: n = 14 per group). (D) Representative images of gastrocnemius muscle cross-sections that were hematoxylin and eosin (HE)-stained or fluorescence immunostained (IF) for myosin heavy <t>chain</t> <t>isoforms</t> ( n = 3 per group).
    Primary Antibodies Against Myosin Heavy Chain Isoforms, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/myosin+isoforms/anti-Myosin+all+isoforms/pmc12864122-60-0-25
    Average 96 stars, based on 1 article reviews
    primary antibodies against myosin heavy chain isoforms - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    96
    Developmental Studies Hybridoma Bank mouse monoclonal anti myosin heavy chain mhc antibody
    Subsets of tenocytes are marked by active Wnt signaling during development. (A,F,K) Ventral views of cranial tendons at 48 hpf (A), 53 hpf (F) and 72 hpf (K) in Tg(scxa:mCherry; 7xTCF:GFP) zebrafish embryos immunostained for anti-mCherry (tenocytes, magenta), anti-GFP (Wnt responsive cells, green) and <t>anti-MHC</t> (muscle fibers, blue). (P) Ventral view of is HCR-stained Tg(7xTCF:GFP) 72 hpf embryo showing expression of scxa in tenocytes (magenta), sox9a in cartilage (blue) and co-immunostained with anti-GFP (Wnt responsive, green). (B-D,G-I,L-N,Q-S) Magnified views of region marked by white dotted lines in A,F,K,P, respectively, showing immunofluorescent signal of mCherry and GFP and is HCR expression of scxa , sox9a and IF staining of GFP. (E,J,O,T) Monochrome panel showing tenocytes co-expressing mCherry and GFP, and scxa and GFP. (U-W) Magnified view of region marked by red dotted lines in P showing is HCR expression of scxa , sox9a and IF staining of GFP. (X) Monochrome image showing tenocytes co-expressing scxa and GFP. Scale bars: 20 μm (A,F,K,Q,U); 30 μm (P); 15 μm (G); 10 μm (B,L).
    Mouse Monoclonal Anti Myosin Heavy Chain Mhc Antibody, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/myosin+isoforms/anti-Myosin+all+isoforms/pmc12891946-243-4-11
    Average 96 stars, based on 1 article reviews
    mouse monoclonal anti myosin heavy chain mhc antibody - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    96
    Developmental Studies Hybridoma Bank anti a4 1025
    Subsets of tenocytes are marked by active Wnt signaling during development. (A,F,K) Ventral views of cranial tendons at 48 hpf (A), 53 hpf (F) and 72 hpf (K) in Tg(scxa:mCherry; 7xTCF:GFP) zebrafish embryos immunostained for anti-mCherry (tenocytes, magenta), anti-GFP (Wnt responsive cells, green) and <t>anti-MHC</t> (muscle fibers, blue). (P) Ventral view of is HCR-stained Tg(7xTCF:GFP) 72 hpf embryo showing expression of scxa in tenocytes (magenta), sox9a in cartilage (blue) and co-immunostained with anti-GFP (Wnt responsive, green). (B-D,G-I,L-N,Q-S) Magnified views of region marked by white dotted lines in A,F,K,P, respectively, showing immunofluorescent signal of mCherry and GFP and is HCR expression of scxa , sox9a and IF staining of GFP. (E,J,O,T) Monochrome panel showing tenocytes co-expressing mCherry and GFP, and scxa and GFP. (U-W) Magnified view of region marked by red dotted lines in P showing is HCR expression of scxa , sox9a and IF staining of GFP. (X) Monochrome image showing tenocytes co-expressing scxa and GFP. Scale bars: 20 μm (A,F,K,Q,U); 30 μm (P); 15 μm (G); 10 μm (B,L).
    Anti A4 1025, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/myosin+isoforms/anti-Myosin+all+isoforms/pm41511973-380-24-26
    Average 96 stars, based on 1 article reviews
    anti a4 1025 - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    96
    Developmental Studies Hybridoma Bank type iib myosin heavy chain isoforms
    Subsets of tenocytes are marked by active Wnt signaling during development. (A,F,K) Ventral views of cranial tendons at 48 hpf (A), 53 hpf (F) and 72 hpf (K) in Tg(scxa:mCherry; 7xTCF:GFP) zebrafish embryos immunostained for anti-mCherry (tenocytes, magenta), anti-GFP (Wnt responsive cells, green) and <t>anti-MHC</t> (muscle fibers, blue). (P) Ventral view of is HCR-stained Tg(7xTCF:GFP) 72 hpf embryo showing expression of scxa in tenocytes (magenta), sox9a in cartilage (blue) and co-immunostained with anti-GFP (Wnt responsive, green). (B-D,G-I,L-N,Q-S) Magnified views of region marked by white dotted lines in A,F,K,P, respectively, showing immunofluorescent signal of mCherry and GFP and is HCR expression of scxa , sox9a and IF staining of GFP. (E,J,O,T) Monochrome panel showing tenocytes co-expressing mCherry and GFP, and scxa and GFP. (U-W) Magnified view of region marked by red dotted lines in P showing is HCR expression of scxa , sox9a and IF staining of GFP. (X) Monochrome image showing tenocytes co-expressing scxa and GFP. Scale bars: 20 μm (A,F,K,Q,U); 30 μm (P); 15 μm (G); 10 μm (B,L).
    Type Iib Myosin Heavy Chain Isoforms, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/myosin+isoforms/anti-Myosin+heavy+chain+Type+IIB/pmc12855016-379-22-28
    Average 96 stars, based on 1 article reviews
    type iib myosin heavy chain isoforms - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    Image Search Results


    Baseline morphological and physiological characteristics of the mice following HRW intake. (A) Body weight (g) of the mice consuming purified water (PW) or hydrogen-rich water (HRW) (PW: n = 12 per group, HRW: n = 14 per group). (B) Forelimb grip strength (g/g), measured weekly during the intervention period (PW: n = 12 per group, HRW: n = 14 per group). (C) Wet masses of six hindlimb skeletal muscles (soleus, extensor digitorum longus (EDL), plantaris, tibialis cranialis (TC), gastrocnemius, and quadriceps femoris) (PW: n = 12 per group, HRW: n = 14 per group). (D) Representative images of gastrocnemius muscle cross-sections that were hematoxylin and eosin (HE)-stained or fluorescence immunostained (IF) for myosin heavy chain isoforms ( n = 3 per group).

    Journal: Frontiers in Nutrition

    Article Title: Hydrogen-rich water improves endurance by reducing skeletal muscle oxidative stress and inflammatory responses

    doi: 10.3389/fnut.2026.1722091

    Figure Lengend Snippet: Baseline morphological and physiological characteristics of the mice following HRW intake. (A) Body weight (g) of the mice consuming purified water (PW) or hydrogen-rich water (HRW) (PW: n = 12 per group, HRW: n = 14 per group). (B) Forelimb grip strength (g/g), measured weekly during the intervention period (PW: n = 12 per group, HRW: n = 14 per group). (C) Wet masses of six hindlimb skeletal muscles (soleus, extensor digitorum longus (EDL), plantaris, tibialis cranialis (TC), gastrocnemius, and quadriceps femoris) (PW: n = 12 per group, HRW: n = 14 per group). (D) Representative images of gastrocnemius muscle cross-sections that were hematoxylin and eosin (HE)-stained or fluorescence immunostained (IF) for myosin heavy chain isoforms ( n = 3 per group).

    Article Snippet: Primary antibodies against myosin heavy chain isoforms were applied overnight at 4 °C (type I (BA-D5), IIa (SC-71), IIx (6H1), or IIb (BF-F3) (all from DSHB, Iowa City, IA, USA)).

    Techniques: Purification, Muscles, Staining, Fluorescence

    Subsets of tenocytes are marked by active Wnt signaling during development. (A,F,K) Ventral views of cranial tendons at 48 hpf (A), 53 hpf (F) and 72 hpf (K) in Tg(scxa:mCherry; 7xTCF:GFP) zebrafish embryos immunostained for anti-mCherry (tenocytes, magenta), anti-GFP (Wnt responsive cells, green) and anti-MHC (muscle fibers, blue). (P) Ventral view of is HCR-stained Tg(7xTCF:GFP) 72 hpf embryo showing expression of scxa in tenocytes (magenta), sox9a in cartilage (blue) and co-immunostained with anti-GFP (Wnt responsive, green). (B-D,G-I,L-N,Q-S) Magnified views of region marked by white dotted lines in A,F,K,P, respectively, showing immunofluorescent signal of mCherry and GFP and is HCR expression of scxa , sox9a and IF staining of GFP. (E,J,O,T) Monochrome panel showing tenocytes co-expressing mCherry and GFP, and scxa and GFP. (U-W) Magnified view of region marked by red dotted lines in P showing is HCR expression of scxa , sox9a and IF staining of GFP. (X) Monochrome image showing tenocytes co-expressing scxa and GFP. Scale bars: 20 μm (A,F,K,Q,U); 30 μm (P); 15 μm (G); 10 μm (B,L).

    Journal: Development (Cambridge, England)

    Article Title: Analysis of cranial tenocyte heterogeneity reveals a role for Wnt signaling in tendon attachments

    doi: 10.1242/dev.205047

    Figure Lengend Snippet: Subsets of tenocytes are marked by active Wnt signaling during development. (A,F,K) Ventral views of cranial tendons at 48 hpf (A), 53 hpf (F) and 72 hpf (K) in Tg(scxa:mCherry; 7xTCF:GFP) zebrafish embryos immunostained for anti-mCherry (tenocytes, magenta), anti-GFP (Wnt responsive cells, green) and anti-MHC (muscle fibers, blue). (P) Ventral view of is HCR-stained Tg(7xTCF:GFP) 72 hpf embryo showing expression of scxa in tenocytes (magenta), sox9a in cartilage (blue) and co-immunostained with anti-GFP (Wnt responsive, green). (B-D,G-I,L-N,Q-S) Magnified views of region marked by white dotted lines in A,F,K,P, respectively, showing immunofluorescent signal of mCherry and GFP and is HCR expression of scxa , sox9a and IF staining of GFP. (E,J,O,T) Monochrome panel showing tenocytes co-expressing mCherry and GFP, and scxa and GFP. (U-W) Magnified view of region marked by red dotted lines in P showing is HCR expression of scxa , sox9a and IF staining of GFP. (X) Monochrome image showing tenocytes co-expressing scxa and GFP. Scale bars: 20 μm (A,F,K,Q,U); 30 μm (P); 15 μm (G); 10 μm (B,L).

    Article Snippet: Embryos were stained using mouse monoclonal anti-Myosin heavy chain (MHC) antibody (Developmental Studies Hybridoma Bank, A4.1025; RRID:AB_528356) at 1:200 dilution, rat monoclonal anti-mCherry antibody (Invitrogen, M11217 ; RRID:AB_2536611) at 1:500 dilution, rabbit polyclonal anti p-Myosin light chain (p-MLC) antibody (Cell Signaling Technology, 3671; RRID:AB_330248) at 1:500 dilution, rabbit anti-Thrombospondin4b antibody (Thbs4b) (GeneTex, GTX125869; RRID:AB_2885605) at 1:1000 dilution.

    Techniques: Staining, Expressing

    Genetic perturbation of Wnt signaling disrupts muscle attachments and tendon pattern. (A-R) Ventral views of cranial tendons at 48 hpf and 60 hpf Tg(scxa:mCherry) , Tg(scxa:mCherry; hsp70l:dkk1b-GFP) and Tg(scxa:mCherry; hsp70l:dnTCF-GFP) embryos that were heat shocked and immunostained for anti-mCherry (tenocytes), anti-GFP (Wnt responsive cells) and anti-MHC (muscle fibers). Images show muscle attachments in untreated (A,J) and heat-shocked controls (B,K), Tg(scxa:mCherry) and heat-shocked transgenics with perturbed Wnt signaling (D-I, M-R). (C,L) Histograms show distribution of normal, mild and severe muscle attachment defects between control and heat-shocked Tg(scxa:mCherry; hsp70l:dkk1b-GFP) embryos. P -values were calculated using chi-square test of independence. *** P <0.001 (48 hpf, n ≈79; 60 hpf, n ≈76). Scale bars: 30 μm.

    Journal: Development (Cambridge, England)

    Article Title: Analysis of cranial tenocyte heterogeneity reveals a role for Wnt signaling in tendon attachments

    doi: 10.1242/dev.205047

    Figure Lengend Snippet: Genetic perturbation of Wnt signaling disrupts muscle attachments and tendon pattern. (A-R) Ventral views of cranial tendons at 48 hpf and 60 hpf Tg(scxa:mCherry) , Tg(scxa:mCherry; hsp70l:dkk1b-GFP) and Tg(scxa:mCherry; hsp70l:dnTCF-GFP) embryos that were heat shocked and immunostained for anti-mCherry (tenocytes), anti-GFP (Wnt responsive cells) and anti-MHC (muscle fibers). Images show muscle attachments in untreated (A,J) and heat-shocked controls (B,K), Tg(scxa:mCherry) and heat-shocked transgenics with perturbed Wnt signaling (D-I, M-R). (C,L) Histograms show distribution of normal, mild and severe muscle attachment defects between control and heat-shocked Tg(scxa:mCherry; hsp70l:dkk1b-GFP) embryos. P -values were calculated using chi-square test of independence. *** P <0.001 (48 hpf, n ≈79; 60 hpf, n ≈76). Scale bars: 30 μm.

    Article Snippet: Embryos were stained using mouse monoclonal anti-Myosin heavy chain (MHC) antibody (Developmental Studies Hybridoma Bank, A4.1025; RRID:AB_528356) at 1:200 dilution, rat monoclonal anti-mCherry antibody (Invitrogen, M11217 ; RRID:AB_2536611) at 1:500 dilution, rabbit polyclonal anti p-Myosin light chain (p-MLC) antibody (Cell Signaling Technology, 3671; RRID:AB_330248) at 1:500 dilution, rabbit anti-Thrombospondin4b antibody (Thbs4b) (GeneTex, GTX125869; RRID:AB_2885605) at 1:1000 dilution.

    Techniques: Control